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pet21a bacterial expression vector  (Millipore)


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    Structured Review

    Millipore pet21a bacterial expression vector
    Bacterial strains and plasmids used in this study
    Pet21a Bacterial Expression Vector, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bacterial+expression+vector+pet21a/pet28a/pmc09841765-94-22-27
    Average 90 stars, based on 1 article reviews
    pet21a bacterial expression vector - by Bioz Stars, 2026-11
    90/100 stars

    Images

    1) Product Images from "Biochemical characterization of bacterial FeoBs: A perspective on nucleotide specificity"

    Article Title: Biochemical characterization of bacterial FeoBs: A perspective on nucleotide specificity

    Journal: Archives of biochemistry and biophysics

    doi: 10.1016/j.abb.2020.108350

    Bacterial strains and plasmids used in this study
    Figure Legend Snippet: Bacterial strains and plasmids used in this study

    Techniques Used: Plasmid Preparation, Clone Assay, Expressing, Mutagenesis

    Related Articles

    Construct:

    Article Title: Primary structure elements of spider dragline silks and their contribution to protein solubility.
    Article Snippet: Spider silk proteins have mainly been investigated with regard to their contribution to mechanical properties of the silk thread.. However, little is known about the molecular mechanisms of silk assembly.. As a first step toward characterizing this process, we aimed to identify primary structure elements of the garden spider’s (Araneus diadematus) major dragline silk proteins ADF-3 and ADF-4 that determine protein solubility.

    Plasmid Preparation:

    Article Title: Primary structure elements of spider dragline silks and their contribution to protein solubility.
    Article Snippet: Spider silk proteins have mainly been investigated with regard to their contribution to mechanical properties of the silk thread.. However, little is known about the molecular mechanisms of silk assembly.. As a first step toward characterizing this process, we aimed to identify primary structure elements of the garden spider’s (Araneus diadematus) major dragline silk proteins ADF-3 and ADF-4 that determine protein solubility.

    Article Title: Characterization of the thermosensitive ts453 reovirus mutant: increased dsRNA binding of sigma 3 protein correlates with interferon resistance.
    Article Snippet: .. Protein expression and Northwestern blot assay Bacterial expression vector pET21a and bacterial Escherichia coli strain BL21(DE3) were originally obtained from Novagen Inc. Plasmids harboring mutated S4 genes under the control of the T7 promoter were transformed in the BL21(DE3) strain expressing the T7 polymerase under the control of an IPTG-inducible promoter. ..

    Article Title: Functionalization of fluorinated benzenesulfonamides and their inhibitory properties toward carbonic anhydrases.
    Article Snippet: Human carbonic anhydrases (CA) are zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide.. This reaction is very important in the human body, as it regulates a broad range of physiological functions such as respiration, CO2/bicarbonate transport between lungs and metabolizing tissues, pH and CO2 homeostasis, and electrolyte secretion in many tissues and organs.. 2] There are 12 active CA isoforms, which differ in cellular localization, distribution in organs and tissues, expression levels, and kinetic properties.

    Expressing:

    Article Title: Primary structure elements of spider dragline silks and their contribution to protein solubility.
    Article Snippet: Spider silk proteins have mainly been investigated with regard to their contribution to mechanical properties of the silk thread.. However, little is known about the molecular mechanisms of silk assembly.. As a first step toward characterizing this process, we aimed to identify primary structure elements of the garden spider’s (Araneus diadematus) major dragline silk proteins ADF-3 and ADF-4 that determine protein solubility.

    Article Title: Characterization of the thermosensitive ts453 reovirus mutant: increased dsRNA binding of sigma 3 protein correlates with interferon resistance.
    Article Snippet: .. Protein expression and Northwestern blot assay Bacterial expression vector pET21a and bacterial Escherichia coli strain BL21(DE3) were originally obtained from Novagen Inc. Plasmids harboring mutated S4 genes under the control of the T7 promoter were transformed in the BL21(DE3) strain expressing the T7 polymerase under the control of an IPTG-inducible promoter. ..

    Article Title: The ATPase Cycle of the Mitotic Motor CENP-E
    Article Snippet: .. The full-length Xenopus xCENP-E cDNA in pBLUESCRIPT was obtained from Dr. Yinghui Mao and was used as a PCR template to create a 342-amino acid length monomer (CENP-E 342) to be inserted into the bacterial expression vector pET21a (Novagen, Madison, WI) between the NdeI and XhoI sites with the following primers: GGAGAATATACATATGTCCGAGGGAGATGCAGTTAA AGTGTGTG and AAGTTGCATGTGCTCGAGATCATCCAGGACCTCATTAACATGGGG. ..

    Article Title: Functionalization of fluorinated benzenesulfonamides and their inhibitory properties toward carbonic anhydrases.
    Article Snippet: Human carbonic anhydrases (CA) are zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide.. This reaction is very important in the human body, as it regulates a broad range of physiological functions such as respiration, CO2/bicarbonate transport between lungs and metabolizing tissues, pH and CO2 homeostasis, and electrolyte secretion in many tissues and organs.. 2] There are 12 active CA isoforms, which differ in cellular localization, distribution in organs and tissues, expression levels, and kinetic properties.

    Sequencing:

    Article Title: Primary structure elements of spider dragline silks and their contribution to protein solubility.
    Article Snippet: Spider silk proteins have mainly been investigated with regard to their contribution to mechanical properties of the silk thread.. However, little is known about the molecular mechanisms of silk assembly.. As a first step toward characterizing this process, we aimed to identify primary structure elements of the garden spider’s (Araneus diadematus) major dragline silk proteins ADF-3 and ADF-4 that determine protein solubility.

    Control:

    Article Title: Characterization of the thermosensitive ts453 reovirus mutant: increased dsRNA binding of sigma 3 protein correlates with interferon resistance.
    Article Snippet: .. Protein expression and Northwestern blot assay Bacterial expression vector pET21a and bacterial Escherichia coli strain BL21(DE3) were originally obtained from Novagen Inc. Plasmids harboring mutated S4 genes under the control of the T7 promoter were transformed in the BL21(DE3) strain expressing the T7 polymerase under the control of an IPTG-inducible promoter. ..

    Transformation Assay:

    Article Title: Characterization of the thermosensitive ts453 reovirus mutant: increased dsRNA binding of sigma 3 protein correlates with interferon resistance.
    Article Snippet: .. Protein expression and Northwestern blot assay Bacterial expression vector pET21a and bacterial Escherichia coli strain BL21(DE3) were originally obtained from Novagen Inc. Plasmids harboring mutated S4 genes under the control of the T7 promoter were transformed in the BL21(DE3) strain expressing the T7 polymerase under the control of an IPTG-inducible promoter. ..

    Polymerase Chain Reaction:

    Article Title: The ATPase Cycle of the Mitotic Motor CENP-E
    Article Snippet: .. The full-length Xenopus xCENP-E cDNA in pBLUESCRIPT was obtained from Dr. Yinghui Mao and was used as a PCR template to create a 342-amino acid length monomer (CENP-E 342) to be inserted into the bacterial expression vector pET21a (Novagen, Madison, WI) between the NdeI and XhoI sites with the following primers: GGAGAATATACATATGTCCGAGGGAGATGCAGTTAA AGTGTGTG and AAGTTGCATGTGCTCGAGATCATCCAGGACCTCATTAACATGGGG. ..

    Article Title: Functionalization of fluorinated benzenesulfonamides and their inhibitory properties toward carbonic anhydrases.
    Article Snippet: Human carbonic anhydrases (CA) are zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide.. This reaction is very important in the human body, as it regulates a broad range of physiological functions such as respiration, CO2/bicarbonate transport between lungs and metabolizing tissues, pH and CO2 homeostasis, and electrolyte secretion in many tissues and organs.. 2] There are 12 active CA isoforms, which differ in cellular localization, distribution in organs and tissues, expression levels, and kinetic properties.

    Clone Assay:

    Article Title: Functionalization of fluorinated benzenesulfonamides and their inhibitory properties toward carbonic anhydrases.
    Article Snippet: Human carbonic anhydrases (CA) are zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide.. This reaction is very important in the human body, as it regulates a broad range of physiological functions such as respiration, CO2/bicarbonate transport between lungs and metabolizing tissues, pH and CO2 homeostasis, and electrolyte secretion in many tissues and organs.. 2] There are 12 active CA isoforms, which differ in cellular localization, distribution in organs and tissues, expression levels, and kinetic properties.



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    Bacterial strains and plasmids used in this study

    Journal: Archives of biochemistry and biophysics

    Article Title: Biochemical characterization of bacterial FeoBs: A perspective on nucleotide specificity

    doi: 10.1016/j.abb.2020.108350

    Figure Lengend Snippet: Bacterial strains and plasmids used in this study

    Article Snippet: : F − omp T hsd S B (r B − , m B − ) gal dcm (DE3) [ 41 ] Plasmids pET21a Bacterial expression vector Novagen pEcNFeoB pET21a carrying the cytosolic domain of E. coli FeoB This study pSmNFeoB pET21a carrying the cytosolic domain of S. mutans FeoB This study pEcNFeoB-N11A pEcNFeoB with a N11A mutation This study pSmNFeoB-N11A pSmNFeoB with a N11A mutation This study Open in a separate window Bacterial strains and plasmids used in this study.

    Techniques: Plasmid Preparation, Clone Assay, Expressing, Mutagenesis